FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341827
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1404.9112714E-1033.693671
CGAGTAG6900.032.0553
CGGAGGT8900.031.6808647
CCGAGTA7400.031.1876342
GCTGGGA60150.030.00079
GAGTAGC18400.029.4121694
TCGCGTT804.747536E-429.4121675
TACTCGG8550.029.1713663
CCCGAGT10400.027.6676851
AGGCGGA11200.027.3112984
CTACTCG8000.027.0823252
CGCGAGT1057.244304E-526.9549351
AGTAGCT33450.026.7303415
GGCGGAG12200.026.61565
GCGGAGG10850.026.4280976
GTAGCTG33650.026.1229556
TAGCTGG36550.025.9716647
GCTACTC17200.025.7795751
TTGGGAG45150.025.403756
CCCAAAG50550.025.3818971
Produced by FastQC (version 0.11.5)