FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341827
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT10700.036.826867
GGCGGAG14900.035.284225
CTACTCG6600.033.4279062
CTCGCGT1309.313226E-932.4973534
GCTGGGA61850.032.3903669
TACTCGG6450.032.0210233
GCGGAGG12550.031.7677086
GCTACTC19200.031.6481911
AGGCGGA16750.030.8266744
GAGTAGC19800.030.8195934
CGAGTAG9100.030.4335693
TCGCGTT1103.0000901E-629.87135
GGAGGCT48700.029.8648579
CCGAGTA9750.029.8498632
TTGGGAG49800.029.6682456
TAGCTGG36000.028.9280827
AGTAGCT34250.028.918165
GTAGCTG34650.028.5620826
GGAGGCG17550.028.352022
CCCGAGT12700.028.1883951
Produced by FastQC (version 0.11.5)