FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552269
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8750.035.4061473
CCGAGTA9550.033.9156952
CTACTCG7850.033.486782
TACTCGG7900.032.0855453
CCCGAGT12000.031.8032171
GCTGGGA60600.031.1217339
GAGTAGC21250.030.4842264
ATGCGCG650.00619357828.8869484
CGGAGGT11150.028.6109397
AGTAGCT34750.028.0972335
TTGGGAG51650.027.6983726
CCCAAAG53350.027.642491
TCGGGAG10450.026.482596
GGGAGGC62200.026.4737138
AGGCGGA16100.026.2404734
GTAGCTG36150.025.950546
GGCGGAG15800.025.8474185
GGAGGCT51300.025.6065469
TAGCTGG38550.025.4343137
GCGGAGG14100.024.949796
Produced by FastQC (version 0.11.5)