FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510544
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG952.0485459E-839.7310141
CCGAACG804.67667E-429.4878621
TCGCGTT650.006109874728.967515
CGAGTAG8400.028.5795523
CGGAGGT7300.028.3246027
CCGAGTA8950.027.8978482
GCTGGGA53850.027.2275859
CTCGCGT1101.0083454E-425.6757474
CTACTCG5950.025.3367462
GGAGGCT41900.024.562379
TACTCGG5600.024.3766773
GGCCGGT3700.024.1315867
CCCGAGT12550.024.0602151
TCGCGGG1201.8352464E-423.5072616
GAGTAGC18900.023.4115734
CGCGGGC1703.4459354E-622.1244816
GGGAGGC48100.021.9825298
TAGCTGG34750.021.7723397
CCCAAAG49850.021.7683721
CCGTGCC6100.021.656661
Produced by FastQC (version 0.11.5)