FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510544
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT959.3054405E-734.7036671
TACTCGG7900.034.463313
CTACTCG7400.034.2555162
CGGAGGT12250.032.1705067
CCGAGTA7100.031.0747762
CGAGTAG7000.030.8472123
GCTACTC20050.029.8325441
GCTGGGA57900.029.2524269
CCCGAGT10200.028.6280961
CTCGCGT1154.240592E-628.573034
TTGGGAG47800.028.5573526
GGAGGCT48950.028.4666969
AGGCGGA16300.027.3584864
GGCGGAG16500.027.3109745
CCCAAAG50300.026.872921
GGGAGGC57700.026.3441358
GGAGGTT34850.026.116428
GCGGAGG14150.025.8577656
CTCGGGA10350.025.851425
GGAGGCG16550.025.2441352
Produced by FastQC (version 0.11.5)