FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177793
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG450.001005760241.8490073
CGAGTAG6700.040.755943
CCGAGTA6850.039.891772
CCCGAGT9350.036.8343961
GCTGGGA61100.034.7638479
GAGTAGC17000.033.786924
CGTCGAG1102.8842387E-630.0225561
TAGCTGG35350.029.644637
AGTAGCT33150.029.2564335
GTAGCTG33050.029.162056
TTGGGAG49400.028.9322576
GGGAGGC53950.028.3088688
CCCAAAG50000.028.3069821
GTGCTGG42100.027.7937977
CGGAGGT11200.026.8529837
GGAGGCT44800.026.5383019
TGCTGGG48400.026.1178578
AGTGCTG44400.026.0488016
GCTACTC16500.026.0195481
AAAGTGC42300.025.821734
Produced by FastQC (version 0.11.5)