FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3177793
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT12650.042.271677
CTACTCG8100.041.722452
TACTCGG8100.041.7191663
GCTACTC20400.040.639351
CGAGTAG7750.040.5752533
CCGAGTA7900.039.8079722
GCTGGGA66350.035.9197359
GCGGAGG15800.035.025546
CCCGAGT9850.034.431861
GGCGGAG18150.034.391915
GAGTAGC19650.033.916734
AGTAGCT34550.033.8245935
TCGGGAG9850.033.8050846
GGAGGCT53300.033.535759
CGTACCG702.2039624E-433.524333
GTAGCTG35900.032.6591956
TAGCTGG38000.031.7237597
CTCGGGA10250.031.5941375
TTGGGAG51800.031.507176
GCCGTAC1501.0459189E-931.4031351
Produced by FastQC (version 0.11.5)