FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4009645
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1500.040.8841741
CGTTGCG702.1390262E-433.6957441
CGGAGGT8550.031.3289627
GCTGGGA64100.028.665279
GAGTAGC23450.028.1069624
CCGAGTA9950.027.9365312
CGAGTAG9900.027.5817283
CCCGAGT12650.027.2229631
CTACTCG6900.026.6292552
AGTAGCT38550.026.501115
TACTCGG7000.026.2298183
CGCGAGT909.318596E-426.2078021
CCCGAAT6250.025.6626821
GGCGGAG12250.024.596465
AGGCGGA11900.024.5286374
GCGGAGG10450.024.2918766
GGGAGGC55600.024.2574088
GTAGCTG39800.024.213326
CCCAAAG58850.024.0479411
TTGGGAG50200.023.9728576
Produced by FastQC (version 0.11.5)