FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3757886
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA3102.5548262E-4120.643936320-329
GTATTCG3702.9631378E-4116.225296340-345
AGTAGCT80950.060.725175
CGAGTAG20300.059.602053
GTAGCTG84900.058.5184146
TAGCTGG91200.055.6335457
CCGAGTA23400.055.2569662
GCTGGGA164500.054.9570669
GAGTAGC52950.053.5964364
CGAATCG1650.001872360851.661167270-279
CCCGAGT26500.049.8007851
GGAGGCT108600.048.7005239
TTGGGAG115500.048.4396366
AAGTGCT91350.048.05615
GCTCGAA5603.3888027E-644.52336320-329
CAAGTAG39500.043.7813763
AGTGCTG98700.043.0264936
GGGAGGC145850.042.9423648
CGGAGGT23600.042.8909037
TAAGCGG3159.773053E-442.567425330-339
Produced by FastQC (version 0.11.5)