FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3757886
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG20000.069.7588653
CCGAGTA21600.067.0981452
AGTAGCT78350.061.8655665
CTACTCG22600.061.468232
GAGTAGC51000.060.255674
CCCGAGT25600.059.3268741
GTAGCTG81550.058.7429926
TACTCGG23800.057.8628653
GCTACTC53450.056.603971
GCTGGGA163700.054.6090939
TTGGGAG124100.054.100736
GGAGGCT128200.053.8808759
TAGCTGG92800.052.1460767
CTCGGGA27250.051.2005355
TCGGGAG30500.050.4500776
AAGTGCT93250.047.4659275
TTCGTTG15400.047.405354245
GGGAGGC180700.045.509468
GCACTTT102000.044.728731
ACTCGGG30150.044.679464
Produced by FastQC (version 0.11.5)