FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626686
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG6600.031.3867443
GCTGGGA63500.030.4160339
CGTCGCG650.006160284428.9188569
CCGAGTA9400.028.068282
GAGTAGC21050.027.9573154
CGAGTAG8800.027.820073
CCCGAGT12050.027.0165791
CTACTCG7500.027.0123772
AGTAGCT34700.026.0500955
CGTCGAG1458.373354E-726.0309661
GGAGGCT46350.025.7524459
GCTACTC18000.025.6875041
GTAGCTG37350.025.1744236
TAGCTGG41150.025.0097147
GGCGGAG12100.024.901885
CGGAGGT10050.024.7824527
GGGAGGC52050.023.9254238
TCGGGAG9550.023.6296886
GCGGAGG11550.023.6083766
CGCGAGT1401.8761324E-523.5905651
Produced by FastQC (version 0.11.5)