FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407573
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6950.034.4471362
TACTCGG7500.033.7987173
CGGAGGT11050.031.8451677
CGCGAGT902.5565556E-531.4025691
CCCGAAT6550.029.4848541
AGGCGGA13450.029.3177814
TCGCGTT1154.239175E-628.5742135
GGAGGCT46150.027.4496369
GCTGGGA58350.027.2585899
GCTACTC18700.027.2043631
GGCGGAG14650.026.5958925
GGAGGCG14600.026.3650572
TCGGGAG10300.025.5034966
GAGTAGC20050.025.2861944
CTCGCGT1501.1759457E-625.0364554
CGAGTAG9750.025.0360873
CCGAGTA9950.025.0046352
CTCGGGA10200.024.8523645
GCGGAGG12600.024.570976
GGGAGGC55200.024.2242498
Produced by FastQC (version 0.11.5)