FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3574888
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7150.036.1132932
TACTCGG8050.035.5734633
CGGAGGT11400.034.1483967
GCTACTC19600.030.7836171
GCTGGGA64500.030.6888189
GGCGGAG16500.029.8734515
GGAGGCT54500.029.2623929
GCGGAGC6300.029.0292666
CTCGGGA10200.028.9948225
GGAGGTT32650.028.2995878
AGGCGGA17600.028.273884
GGAGGCG16900.027.7794592
TTGGGAG54450.027.6451636
GGGAGGC65350.027.560228
CCCAAAG54200.027.395361
CGAGTAG8450.027.2227253
TCGACGG700.00888878926.8258933
GTAGCTG34400.026.4457136
AGTAGCT34100.026.431815
GAGTAGC21350.025.9463564
Produced by FastQC (version 0.11.5)