FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2964989
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9150.050.4150543
CCGAGTA9350.049.880532
CCCGAGT11800.044.3800431
GCTGGGA74200.040.7233969
GAGTAGC24850.040.5361634
AGTAGCT39250.040.0554925
GTAGCTG41300.037.566956
CCCAAAG52150.035.644181
TAGCTGG44650.035.258217
TACTCGG7000.034.294733
AAAGTGC45400.033.1779374
AAGTGCT45950.032.678375
CTACTCG6500.032.6141972
TTGGGAG56900.032.4729926
GTGCTGG47800.032.0498967
CCAAAGT51300.031.957252
GGAGGCT51800.031.75239
CGGAGGT10250.031.6345847
GCTACTC19850.031.6109711
AGTGCTG48300.031.5384186
Produced by FastQC (version 0.11.5)