FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705189
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1450.039.0379941
ACGATCG550.002719681534.1769568
GCTGGGA57400.028.081379
CGGAGGT9350.027.1405247
AGGCGGA9800.025.4542544
TACTCGG6450.024.8101713
GGGAGGC49050.023.5685128
CTACTCG6500.023.1896212
CCGAGTA7150.023.0578632
GTGCTGG40200.023.0290627
GGAGGTT28900.022.9275328
CGAGTAG7150.022.3812053
GCGGAGG10900.021.9954136
CTCGGGA8800.021.9286425
CCCAAAG52700.021.7505341
GGAGGCT42850.021.604869
GGCGGAG11350.021.563445
TAGCTGG36650.021.5412737
CCCGAGT11350.021.195741
GTAGCTG33850.021.109286
Produced by FastQC (version 0.11.5)