FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705189
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7900.035.661512
CGGAGGT10500.034.8521047
CGAGTAG7850.033.4956253
AGGCGGA15000.032.2415354
GGCGGAG15200.032.1257745
CCCGAGT10500.031.8658711
ATTCGCG600.004182293531.302464
TACTCGG7450.030.252043
GCGGAGG12450.030.1437596
GCTGGGA59200.030.0367499
CTACTCG7650.028.8477542
GGAGGCG16150.027.329452
GAGTAGC17900.027.2803554
CTCGCGT1057.475936E-526.8306794
TCGCGTT700.00888168426.8303185
GGAGGTT33250.026.8093098
GGAGGCT52100.026.3854989
CCCAAAG51850.025.9031661
AGTAGCT32600.025.204865
TCGGGAG9900.025.1140926
Produced by FastQC (version 0.11.5)