FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921996
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8700.053.9552573
CTACTCG8350.052.8474662
CGAGTAG8700.050.717943
GCTACTC21000.049.14391
CCGAGTA9450.047.6894342
GAGTAGC22450.045.5819784
GCTGGGA80150.044.0156449
TAGCTGG42700.041.9689647
CCCGAGT12500.041.8463671
AGTAGCT39200.041.7919245
GTAGCTG40200.040.4875836
TTGGGAG59150.039.4905366
GGAGGCT63700.038.3699349
TCGGGAG14500.037.523946
CTCGGGA13800.037.4167985
AAAGTGC45300.037.3041654
CCCAAAG57450.036.9940031
CGGAGGT14100.036.266047
ACTCGGG13600.036.241274
AAGTGCT48000.035.30365
Produced by FastQC (version 0.11.5)