FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573516
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7650.043.115062
TACTCGG7450.042.341853
TCGCGTT901.29512046E-841.850325
CGAGTAG10550.041.5032163
CCGAGTA11350.039.4384772
GAGTAGC25550.038.3286674
GCTGGGA80150.036.468439
CCCGAGT13350.036.0538371
GCTACTC19900.035.3318021
AGTAGCT42400.034.5339135
GTAGCTG44750.032.6700256
TAGCTGG46250.031.9042157
CCCAAAG64150.031.8510551
CTCGCGT1201.6289778E-731.3877414
GGAGGCT57750.031.002969
GTGCTGG51400.030.5360977
AAAGTGC51150.029.8229544
ACTCGCG1303.2846765E-728.9732973
TCGGGAG11550.028.8973796
AAGTGCT53450.028.8039045
Produced by FastQC (version 0.11.5)