FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573516
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9900.043.616052
TACTCGG10200.041.8742563
GCTACTC26050.040.514451
TCGCGAA608.46878E-539.44367694
GGAGGCT63100.037.1714369
GAGTAGC22150.036.2349974
GCTGGGA78100.034.537079
CGAGTAG9850.034.3085333
CGGAGGT15600.033.07557
TTGGGAG67100.032.9906546
CTCGCGT1151.1521661E-732.6511154
TCGGGAG13550.032.535696
AGTAGCT39350.032.3234945
CTCGGGA14150.031.84265
CCCAAAG62450.031.762841
CCGAGTA11200.031.4294642
TCGCGTT902.62023E-531.2897785
ACTCGGG14150.030.8483834
GTAGCTG40700.030.4215246
CCCGAGT14600.029.689621
Produced by FastQC (version 0.11.5)