FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3170133
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7600.034.076123
CGCGAGT702.1299391E-433.7199061
CCGAGTA8500.032.717012
GCTGGGA55050.030.9011489
CCCGAGT10400.030.4127641
TACTCGG5400.029.6473713
GAGTAGC17850.028.4896164
CTACTCG5900.027.9612482
GCTACTC16050.027.942351
GGCCGGT2700.027.8470767
TCGCGTT856.745427E-427.6982385
TAGCTGG31600.027.5111057
CGGAGGT8400.026.8525377
GGGAGGC47500.026.8101398
GGAGGCT43950.026.4095769
TTGGGAG43450.026.2937346
GGCGGAG10950.026.2311175
GTGCTGG36500.025.7490087
CGATTGG1659.162977E-825.6838193
AGTAGCT29650.025.2507025
Produced by FastQC (version 0.11.5)