FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3170133
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCG1101.8208084E-938.407773
TCGCGTT1101.8208084E-938.406565
CCGAGTA7200.036.511672
GGCGGAG13800.035.3760875
CGGAGGT10850.034.592027
CGAGTAG7250.034.3168263
CTACTCG6350.034.0063632
GCGGAGG11250.033.7737776
AGGCGGA14750.033.416934
TACTCGG6950.033.0963863
GAGTAGC16200.032.4543044
CTCGCGT1351.344597E-831.295224
GGAGGCT46500.030.8748389
GGAGGCG15950.030.6089742
GCTGGGA57000.030.3730019
GGAGGTT30850.029.198588
CCCGTTA1303.267087E-728.990881
CCCGAGT10100.028.4526841
AGTAGCT31250.028.3901275
GCTACTC17000.028.266111
Produced by FastQC (version 0.11.5)