FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3454286
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1253.6379788E-1241.5187341
CGAGTAG7900.028.0054443
TACTCGG6350.027.4283493
CCGAGTA8250.027.410172
CGCGAGT1257.794672E-626.4210111
CGTGTCG1151.3560575E-424.5797732
GGAGGCT44200.024.4531529
GGGAGGC48200.023.983768
TTGGGAG43500.023.7770276
GCTGGGA61650.023.5534559
GAGTAGC20300.023.4205464
CTACTCG6850.023.3836772
TAGCTGG35700.022.640687
GTAGCTG35250.022.139776
AGTAGCT34500.021.558065
CTCGCGT1100.0030333521.3968074
CCCGAGT12000.021.231171
CCCAAAG51100.021.1434571
GCTACTC18150.021.0557061
CGGAGGT8050.021.0153127
Produced by FastQC (version 0.11.5)