FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3158781
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG8500.038.221933
TACTCGG7050.038.0687143
CGGAGGT10850.037.680087
CTACTCG7650.035.729592
GCTGGGA75400.035.648979
GAGTAGC21550.035.6141664
GCTACTC18100.035.4620171
TCGCGTA401.1608559E-535.24386676-77
CCGAGTA9800.035.1041342
GTAGCTG37750.033.251346
CCCGAGT11600.032.9556661
AGTAGCT38250.032.620975
CGCGAAC600.00410785131.4173972
CCCAAAG56850.031.2978121
TAGCTGG42350.030.6251327
AAAGTGC45250.030.3841274
GGCGGAG14900.030.3366375
GCGGAGG12950.030.1316666
GTGCTGG47750.029.7204827
TCGGGAG10000.029.6179756
Produced by FastQC (version 0.11.5)