FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169211
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5050.042.753353
CTACTCG5850.034.501412
GCTGGGA54900.029.902779
TTCGCGG804.820772E-429.3353025
CCGAGTA7050.029.2946192
CGGAGGT8700.029.1113827
GGCGGAG12250.027.9703165
GGGAGGC48150.027.4693248
CGAGTAG6850.027.4077363
CCCAAAG48900.026.9758191
CCCGAGT10000.026.8534621
TCGCGTA350.00890499326.8161950-51
TCGGGAG7900.026.7119266
GGAGGCT43450.026.3399989
GCGGAGC4850.026.106096
CTCGGGA8300.025.4474935
AGGCGGA12000.025.4239314
GTGCTGG36750.025.3970957
AAAGTGC42200.025.2478264
TGCTGGG42100.024.9552028
Produced by FastQC (version 0.11.5)