FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587016
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10700.039.1749272
CGAGTAG10350.039.1022953
CGTCGAG1450.039.0288621
CGGAGGT11550.038.245847
CCCGAGT14400.035.0424081
GAGTAGC25650.033.574374
GCTGGGA74100.032.5340049
GGCGGAG14600.031.9099795
CTACTCG6800.031.1677572
GCTACTC17850.030.6473141
AGTAGCT40950.030.568345
GTAGCTG39700.030.550546
AGGCGGA15300.029.834894
TACTCGG7550.029.2951033
TAGCTGG43650.029.1758387
GCGGAGG13600.029.0355516
ACGATCG650.00616000828.9191138
TTGGGAG56500.028.2059636
GGAGGCT53000.028.0188039
CCCAAAG55650.027.8806821
Produced by FastQC (version 0.11.5)