FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109596
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT7550.034.2340557
CCGAGTA7750.032.821272
GCTGGGA54000.032.112589
TCGCGTC600.00413556931.3742435
CGAGTAG7500.030.7467613
GAGTAGC18500.029.7631344
TAGCTGG32200.029.3347767
CGTCGAG1503.578498E-828.2987921
CCCGAGT9350.028.2483481
AGTAGCT32050.028.045935
GTAGCTG31850.028.0426756
CCCAAAG44550.026.8907341
GGCGGAG9850.025.800145
GTGCTGG37550.025.2804037
TACTCGG5850.024.9385033
CCGGTAG950.00130605424.7336949
GGAGGCT39450.024.5393379
TTGGGAG45200.024.4401976
GCGGAGG8300.024.3537396
AGGCGGA10050.024.3501594
Produced by FastQC (version 0.11.5)