FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109596
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT10400.039.243997
GGCGGAG13900.035.795625
GCGGAGG12600.033.501286
AGGCGGA14050.033.4089284
GCTACTC17150.031.859451
GCTGGGA55250.031.5038649
GAGTAGC16500.031.293034
GTTCGCG600.004188361631.293033
ACTCGCG902.6182614E-531.2930283
CTACTCG6850.030.836692
GGAGGCT45300.030.7595849
TACTCGG7100.030.4115353
CGAGTAG8400.029.6166153
CCGAGTA8450.029.4418452
TTGGGAG46300.029.0730366
AGTAGCT28650.028.99935
TCGCGGT650.006194588728.8858725
GGAGGTT32700.028.6925748
CCCAAAG47250.028.3113731
CCCGAGT10000.028.2615261
Produced by FastQC (version 0.11.5)