FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568003
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7250.038.1946333
CTACTCG7900.033.8643072
CGGAGGT12100.031.4001857
GGCGGAG16050.030.996535
GCTACTC18850.029.73961
GCGGAGG14200.029.7248556
AGGCGGA16750.029.1411634
GCTGGGA61850.028.7454179
CTCGGGA9950.027.829875
GGAGGCT48450.027.7879969
CCGAGTA8300.027.7084622
TCGGGAG10700.027.1752476
GGAGGTT35550.027.0491078
CGCCGTA1057.091023E-527.03983394
TTGGGAG52200.026.8637286
CGAGTAG8500.025.9517863
CCCAAAG51950.025.8439451
CCCGAGT10650.025.6553461
ATACCGG1101.0338978E-425.581396
GGAGGCG17250.025.5760332
Produced by FastQC (version 0.11.5)