FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3522503
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1457.166818E-1032.5314941
CGAGTAG7050.029.373723
TACGGCG650.00611463128.96295
CCGAGTA7850.027.6040422
TACTCGG6650.027.601863
CCCGAGT10350.026.8895551
GCTGGGA55500.026.418199
CGCGAGT950.001277840824.8266641
TCGCGTT950.001294697324.77095
GAGTAGC19900.024.1236214
CTACTCG7600.023.553452
CCGTACG500.001760901523.49694872-73
AGTAGCT33250.023.0723255
GGAGGCT42950.022.9772249
GTAGCTG34150.022.715046
CCCGAAT6500.022.4967781
GGGAGGC47350.022.3307848
GCTACTC18000.022.0129761
CTCGCGT1503.2590695E-521.9635314
CCCAAAG49000.021.9487951
Produced by FastQC (version 0.11.5)