FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386230
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1256.0499588E-933.967011
CGAGTAG7200.032.684843
CCGAGTA7850.031.8086782
CCCGAGT9750.030.4832171
CGGCCTA2101.8189894E-1229.0910288
GCTGGGA58050.028.333549
TAGCTGG36400.028.0151777
GAGTAGC18600.027.5817894
AGTAGCT32600.026.9980815
TGCGCGG1057.355003E-526.8949575
TGCGACG700.00884464326.8532588
CGGAGGT8800.026.7006847
TTCGGCG909.443647E-426.1478735
CCGGGTT5900.025.4878378
GGAGGCT41750.025.4382369
CGCGAGT1301.0574669E-525.4026811
GTAGCTG35050.024.9460096
TACTCGG6300.024.6537113
CCCGGGT5550.024.5550067
TTGGGAG45600.024.1227686
Produced by FastQC (version 0.11.5)