FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386230
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT10250.034.313477
CTACTCG7100.033.050582
TACTCGG7450.032.755843
TCGCGTT1052.0911993E-631.2864915
AGGCGGA13650.030.9426844
GGCGGAG13850.030.495865
GCTGGGA60200.030.1499529
GCTACTC17700.029.544941
GGAGGCT48800.029.3124549
CCCGAGT10450.028.8534071
CCGAGTA8000.028.7457392
GCGGAGG12850.028.0968196
CCCAAAG50500.028.080741
TCGGGAG9050.027.9778756
GAGTAGC18700.027.3547674
GGGAGGC56050.027.2756848
TTGGGAG50400.027.0727236
CGAGTAG7850.026.9023
CTCGCGT1755.6516E-926.8169924
GGAGGTT30750.025.9261178
Produced by FastQC (version 0.11.5)