FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3554882
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG7900.041.5844573
GCTGGGA74950.039.1176579
GCTACTC19700.038.7380871
CTACTCG8050.038.4792372
CGGAGGT15650.036.8669247
CGAGTAG9700.036.7707023
GAGTAGC23150.036.4911584
CCGAGTA10450.035.4804572
TCGCGTT801.1709411E-535.1987655
TAGCTGG43650.034.710787
GCGGAGG17700.034.184246
CGTACCG550.002737408334.1316573
GTAGCTG42600.033.9117776
AGTAGCT41350.033.4820985
TTGGGAG64900.033.3169866
GTGCTGG49650.032.783587
CCCAAAG61850.032.674351
GGAGGCT61950.032.4090969
CCCGAGT12950.032.374991
GGAGGTT42600.031.9326178
Produced by FastQC (version 0.11.5)