FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363982
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9550.034.40172
CGAGTAG9450.031.7848723
CCCGAGT12900.030.6783431
GCTGGGA66050.028.7617669
CGGAGGT11250.027.9337847
GAGTAGC22750.027.231084
TTGGGAG47800.025.9996456
AGTAGCT36300.025.2115945
GGAGGTT31800.024.9272028
GTAGCTG38100.024.3719146
GGAGGCT50250.023.6166259
TACTCGG9400.023.4661773
CCGGGTT6250.023.2646058
CCCAAAG54500.023.2540251
GGGAGGC59200.023.0560688
TAGCTGG41950.022.6971657
GGCGGAG14450.022.4106045
AGGCGGA14700.022.029474
GTGCTGG44100.022.0160457
GAGGTTG40850.021.931829
Produced by FastQC (version 0.11.5)