FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612970
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1600.056.033871
CGGAGGT10150.028.7051567
TACTCGG7050.026.0416053
CTACTCG7350.025.641912
CCGAGTA10000.024.972022
GCTACTC19550.024.8603611
TCGCGTT950.001293022324.7764245
TTGGGAG48250.024.2621176
CGAGTAG9950.024.1290023
GCTGGGA63950.023.147519
GAGTAGC22700.022.8117754
CTCGCGT1252.3987278E-422.59614
AGGCGGA12000.022.3607234
GGGAGGC56900.021.8860768
CCCAAAG54000.021.6707971
GGAGGCT45400.021.6333989
AGTAGCT35400.021.5428925
GCGGAGG12150.021.2820326
CCCGAGT14200.021.2671171
GGCGGAG12700.021.1282425
Produced by FastQC (version 0.11.5)