FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612970
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT858.036295E-944.173775
TACTCGG7800.036.1035653
CTACTCG7550.034.191742
ACTCGCG1457.566996E-1032.368713
CGGAGGT10550.031.1216287
CTCGGGA9950.029.2456995
CGCGTTG1303.4012737E-728.8580636
GCTGGGA66900.027.3450859
GGCGGAG14450.026.9589925
GCTACTC20550.026.5970541
AGGCGGA14550.026.4511344
GGAGGCT49350.025.8536919
GCGGAGG12850.025.5455636
GTGCTGG42600.025.4342617
TCGGGAG11250.025.0103236
GGAGGTT32700.024.5281688
CTCGCGT1751.6705235E-724.1378124
CCCAAAG55650.024.1305391
AGTAGCT36900.023.2765255
CGAGTAG8900.023.2036383
Produced by FastQC (version 0.11.5)