FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600008
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6200.032.670312
CTCGCGT1501.0550139E-931.3808654
TACTCGG6050.031.121523
ACTCGCG1401.8655555E-830.260123
CGGAGGT10150.030.0943587
CCGAGTA8400.029.1608772
CGAGTAG8100.029.0563563
TCGCGTT1354.5782508E-727.8941025
CGTCGAG1904.292815E-1027.308791
GAGTAGC20950.026.7373964
GCTGGGA60700.026.3999739
CCCGAGT11100.026.3470571
GCTACTC16600.026.142261
AGTAGCT35950.025.5324155
TAGCTGG39250.024.903567
GTAGCTG36150.024.8423186
TTCGGCG1000.00174395523.535655
GGGAGGC54650.023.3892618
TTGGGAG49250.022.6260726
CCCGAAT6900.022.5594371
Produced by FastQC (version 0.11.5)