FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3847223
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8400.038.5625843
CTACTCG8400.037.4453122
GCTACTC22500.034.3312451
CGGAGGT13850.032.8550347
CGAGTAG8250.031.2971673
GCTGGGA70950.031.0792549
GAGTAGC21150.030.187344
GGAGGCT60650.029.2405919
GGCGGAG17750.028.8275345
TCGGGAG12550.028.0304286
ATGCGCG856.8652065E-427.6151494
GGGAGGC68600.027.5591968
TTGGGAG59650.027.4427246
GCGGAGG15950.027.3485626
CCGAGTA10000.027.228892
AGTAGCT36450.026.78835
AGGCGGA19350.026.2022824
CCCAAAG63050.026.1463581
GTGCTGG49250.025.813277
CTCGGGA12750.025.7731345
Produced by FastQC (version 0.11.5)