FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3767832
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11350.039.0068782
CGAGTAG10750.038.9669343
GAGTAGC23500.034.6491784
CCCGAGT15550.034.579561
GCTGGGA69200.033.0717359
CGTCGAG1900.032.272661
AGTAGCT41550.030.4716455
TAGCTGG41950.030.3578787
CTACTCG7150.030.301282
GTAGCTG42250.028.2608476
GCTACTC20300.027.6500511
CGGAGGT11250.027.5692847
GGAGGCT52650.027.0444629
GGGAGGC58450.026.85328
TTGGGAG55950.025.9619086
CCCAAAG60500.025.1041491
TACTCGG8700.024.8858953
TCGCGGG1151.3762925E-424.5262786
GTGCTGG47300.024.4403867
GGAGGTT31950.023.9745718
Produced by FastQC (version 0.11.5)