FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3767832
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9700.044.51973
CTACTCG9350.043.6778342
GCTACTC22500.037.268831
CGAGTAG9050.036.3066023
CGGAGGT13800.034.3344737
CTCGGGA12550.034.0356335
CGGTACG702.2020943E-433.5293732
GCTGGGA73150.033.414479
GGAGGCT61400.033.008569
CCGAGTA10150.032.3731882
GAGTAGC23350.031.3598424
CCCGAGT12300.031.0233151
TTGGGAG62800.030.6214566
AGTAGCT37050.030.5326825
TAGCTGG42150.030.161987
TCGGGAG13900.030.0314886
GGGAGGC75400.029.5539918
ACTCGGG14750.028.9591314
CCCAAAG59600.028.7715591
GTAGCTG40200.028.585236
Produced by FastQC (version 0.11.5)