FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876014
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10900.032.8506662
CGTCGAG1755.456968E-1232.3499451
CGAGTAG10850.031.2372263
TACTCGG7650.028.920523
CCCGAGT13900.028.849251
GCTGGGA69650.028.809549
CTACTCG8000.027.679932
CCCGAAT6150.026.0815961
CGGAGGT9500.025.722277
GAGTAGC24850.025.1938724
AGTAGCT39600.025.08175
TTGGGAG53050.024.2801636
GGAGGCT52650.024.0095189
GTGCTGG46150.023.8272367
TAGCTGG45500.023.7545057
CCCAAAG56900.023.5470491
GTAGCTG40950.023.5334936
GGGAGGC59800.023.417728
TCGGGAG12300.022.5493136
CTCGGGA11900.022.5474575
Produced by FastQC (version 0.11.5)