FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551508
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2250.046.1217541
CGAGTAG7950.030.7841453
CGCGAGT804.6828415E-429.4812341
CCGAGTA8750.029.0732252
ACGTCGA1554.9145456E-827.3890171
CTACTCG8400.024.676352
GCTGGGA69250.024.3618839
TACTCGG8550.023.6697273
TCGTACG500.00176102723.49666436-37
GGAGGCT49450.023.4729049
CGGAGGT10050.023.3797657
GAGTAGC22600.023.3238624
GCTACTC20400.022.1976341
AGTAGCT37850.021.8845545
CCCGAGT12950.021.8548151
CCCAAAG56200.021.6545431
GGGAGGC57650.021.0308388
GTGCTGG44100.020.9924857
GTAGCTG37950.020.9345666
TAGCTGG40250.020.8988957
Produced by FastQC (version 0.11.5)