FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551508
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8050.033.2432982
TACTCGG8200.033.2063333
GCTACTC21450.028.9888461
GCTGGGA69100.028.1794229
GAGTAGC23600.028.0487734
GGAGGCT56150.026.4058029
CCGAGTA10750.026.204022
CGAGTAG9750.026.0013523
AGTAGCT37950.024.9888555
TTGGGAG56400.024.7011456
CTCGGGA11700.024.476585
GTAGCTG39550.024.1948766
TCGGGAG12650.023.7317166
GGGAGGC70600.023.5233638
CGGAGGT12000.023.4569137
CCCGAGT14300.023.3887271
TAGCTGG41450.023.315447
AGGCGGA15650.023.0984674
CCCAAAG53650.022.6534461
AAAGTGC45350.022.1535454
Produced by FastQC (version 0.11.5)