FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746147
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10400.041.1943633
CCGAGTA11250.039.7850882
CCCGAGT15000.036.4850541
TACTCGG8050.035.090143
CTACTCG8250.034.8357472
GCTGGGA81650.034.819499
GAGTAGC27200.034.790194
AGTAGCT44250.032.6629075
TAGCTGG47950.032.6346447
GTAGCTG45400.031.5804276
CGTCGAG1351.2805685E-831.4526311
GCTACTC21200.030.4882811
CGGAGGT13050.029.8875857
GGAGGCT60750.028.9300089
CCCAAAG62250.028.4968411
TTGGGAG66300.027.4391986
AAAGTGC50400.027.2760854
GGGAGGC75150.027.0133238
GTGCTGG51750.026.6060547
CCCGAAT4800.026.538161
Produced by FastQC (version 0.11.5)