FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746147
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10850.043.2658843
CCGAGTA11500.041.230832
GAGTAGC27950.040.3092544
CTACTCG9400.039.4546172
CGGAGGT15350.038.8133167
TACTCGG9450.037.7534943
GCTACTC25200.037.38711
CCCGAGT14550.035.937871
TAGCTGG47600.035.7754447
GCTGGGA87550.035.4218529
AGTAGCT46650.035.4214555
GGAGGCT67000.033.7518969
GTAGCTG48950.033.5402226
CCCAAAG67700.033.1911281
TCGCGTT1001.4256275E-632.8604435
GGCGGAG22100.032.7117545
TTGGGAG66600.032.1878936
AGGCGGA23700.031.4937364
GGAGGTT44250.030.5330648
GTGCTGG52550.030.3522957
Produced by FastQC (version 0.11.5)