FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3256861
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT9150.038.45067
TACTCGG6700.035.7277073
GCGGAGG10850.033.7194986
ACTCGCG702.203329E-433.5263
CTACTCG7300.033.4356882
CCGAGTA7500.031.2923762
GGCGGAG13050.031.2909365
AGGCGGA14350.030.7457944
CGAGTAG7500.030.6651153
CCCGAGT9900.029.0310381
GCTACTC18650.028.5474641
GGAGGCT47300.028.3666369
GGAGGCG15100.027.6657772
GCTGGGA55700.027.0366469
GGGAGGC53500.026.6552498
GGAGGTT30850.026.1538878
CCGATAG1301.0680205E-525.3701251
TTGGGAG46850.025.3295336
CTCGGGA9600.024.9349655
AGTAGCT30300.024.320185
Produced by FastQC (version 0.11.5)