FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247760
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG13650.058.803522
TACTCGG14400.058.019943
CGAGTAG14150.057.054743
CCGAGTA14300.055.4741332
GCTACTC31050.053.701931
AGTAGCT53050.047.5140465
CCCGAGT17950.046.9717561
GCTGGGA109200.046.744669
GAGTAGC34000.046.5247574
GTAGCTG54650.045.8321046
TAGCTGG57700.045.2048037
ACTCGGG18300.043.6044654
TCGGGAG19800.043.1146556
CCCAAAG72350.043.099051
GGAGGCT94900.042.9614839
AAAGTGC59650.042.492914
CTCGGGA18800.042.444775
AAGTGCT63100.040.9878735
CCAAAGT66100.040.761512
TTGGGAG86700.040.467026
Produced by FastQC (version 0.11.5)