FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850091
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG1502.7284841E-1134.5146451
CGAGTAG5950.024.4857793
CTACTCG4950.022.8023762
CCGAGTA7000.021.4993822
TACTCGG5650.019.9632843
GAGTAGC15750.019.3955424
GCTGGGA56450.019.3149329
CCCAAAG42050.019.2514951
CGACTAT1250.00636214218.826171
GCGTAGA1250.00636214218.826171
GCTACTC14350.018.6949791
CCCGAGT9900.018.5409261
TAGCTGG30200.017.8961687
GCGTATT1851.633283E-417.808541
AGTAGCT26350.017.6572785
AAAGTGC37000.016.766464
AAGTGCT39350.016.7206235
CCAAAGT41850.016.631842
TCGGGAG9150.016.4360716
GTAGCTG28600.016.432486
Produced by FastQC (version 0.11.5)