FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850091
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG5800.025.9350092
CGAGTAG6650.025.447513
TACTCGG6000.025.0705073
CGACGGT950.001304961324.737169
CCGAGTA7150.024.3254052
CCCGAGT9600.021.0564171
GCTGGGA55500.019.3930429
CCCAAAG45300.018.7831151
GAGTAGC16650.018.6334864
GGGAGGC45050.018.1533978
GGAGGCT44650.017.7897039
TTGGGAG37200.017.5639026
AGTAGCT26800.017.5396945
TAGCTGG29700.017.249387
GTAGCTG29700.016.934666
GTGCTGG40100.016.409197
AAAGTGC40400.016.405984
CCAAAGT44950.016.3139572
GCCGCTA1750.00232654416.1176031
AAGTGCT40950.015.7261875
Produced by FastQC (version 0.11.5)