FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534032
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5950.039.489523
CCGAGTA6600.037.043422
CTACTCG5950.035.558812
TACTCGG5900.034.24883
GAGTAGC16150.032.0072944
TTGGGAG37500.031.3283566
CGTAACG600.00416515831.3283524
CGTCGAA600.00416515831.32835294
CCCAAAG43100.030.6741351
GCTGGGA57200.030.0686479
CCCGAGT8300.030.0428451
CGTTGCG650.006124779628.952761
GTAGCTG27950.028.4140876
GCTACTC15400.028.4122451
AAAGTGC33850.028.3204614
TAGCTGG29650.028.2113677
AGTAGCT27700.027.8222925
GGAGGCT43700.027.3137389
CGGAGGT9050.027.0012347
GGGAGGC48400.026.9915778
Produced by FastQC (version 0.11.5)