FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534032
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG6200.047.7684062
TACTCGG6500.043.394013
GCTACTC15150.042.822841
CCCGTTA906.181799E-736.5649151
CGAGTAG6050.036.52033
TCGGGAG9400.036.006376
TTGGGAG44550.035.9816746
GCTGGGA58350.034.8010149
GAGTAGC16800.034.1369934
CTCGGGA9600.033.7872285
CCCAAAG43550.033.680261
AGTAGCT26550.033.6406065
GGAGGCT51100.033.1154449
GGGAGGC58300.032.8958138
TAGCTGG30450.032.2619677
GTAGCTG27050.031.6285176
CGGAGGT11100.031.3357877
AAAGTGC33850.031.2462984
CCGAGTA6650.031.1044772
GGAGGTT30800.030.2178448
Produced by FastQC (version 0.11.5)